Comparison of API ZYM system with API AN-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile

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Comparison of API ZYM system with API AN-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile.

The API ZYM system was compared with four anaerobe identification systems for the definitive identification of Clostridium difficile by using 88 cultures of C. difficile grown on Mueller-Hinton blood agar medium. The API ZYM system yielded a distinct and consistent enzyme profile for all test strains, whereas the sensitivities of the other systems in identifying C. difficile ranged from 78 to 9...

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API ZYM and API An-Ident reactions of fastidious oral gram-negative species.

API ZYM and API An-Ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "Bacteroides forsythus, the "fusiform" Bacteroides species (A. C. R. Tanner, M. A. Listgarten, M. N. Strzempko, and J. L. Ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these se...

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Identification of Staphylococcus species with the API STAPH-IDENT system.

The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline phosphatase, urease, beta-glucosidase, beta-glucuronidase, and beta-galactosi...

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Use of the API-ZYM system in rapid identification of alpha and non-haemolytic streptococci.

The API-ZYM method of detecting enzymes was tested using 99 streptococci isolated from clinical material and 14 type species obtained from the National Collection of Type Cultures. We found the method easy and reliable. The results obtained indicate that this method could be a useful identification system in busy routine clinical laboratories.

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Rapid identification of thermophilic Naegleria, including Naegleria fowleri using API ZYM system.

The suitability of the API ZYM system for identifying thermophilic Naegleria species, based on enzyme presence and activity, was investigated. Replicate testing on strains of N fowleri, N lovaniensis, and N australiensis cultured in a monoxenic and an axenic medium showed that the system could provide a rapid and reproducible means of identifying the species soon after primary isolation. No sin...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1988

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.26.1.144-146.1988